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A Watershed and Active Contours Based Method for Dendritic Spine Segmentation in 2-Photon Microscopy Images
Authors: Ertunç Erdil, A. Özgür Argunsah, Devrim Ünay, Müjdat Çetin
Published in: SIU 2013
Publication year: 2013
Abstract: Analysing morphological and volumetric properties of dendritic spines from 2-photon microscopy images has been of interest to neuroscientists in recent years. Developing robust and reliable tools for automatic analysis depends on the segmentation quality. In this paper, we propose a new segmentation algorithm for dendritic spine segmentation based on watershed and active contour methods. First, our proposed method coarsely
segments the dendritic spine area using the watershed algorithm. Then, these results are further refined using a region-based active contour approach. We compare our results and the results of existing methods in the literature to manual delineations of a domain expert. Experimental results demonstrate that our proposed method produces more accurate results than the existing algorithms pro-posed for dendritic spine segmentation.

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